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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
22/11/2018 |
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Data da última atualização: |
30/11/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CURSINO, A. E.; VILELA, A. P. P.; FRANCO-LUIZ, A. P. M.; OLIVEIRA, J. G. de; NOGUEIRA, M. F.; ARAÚJO JÚNIOR, J. P.; AGUIAR, D. M. de; KROON, E. G. |
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Afiliação: |
ANDREIA ELISA CURSINO, UFMG; ANA PAULA PESSOA VILELA, UFMG/UFSC; ANA PAULA MOREIRA FRANCO-LUIZ, UFMG/VIRIONTECH DO BRASIL INDÚSTRIA DE INSUMOS E SERVIÇOS EM BIOTECNOLOGIA; JAQUELLINE GERMANO DE OLIVEIRA, FIOCRUZ-MINAS; MARCIA FURLAN NOGUEIRA T DE LIMA, CPAP; JOÃO PESSOA ARAÚJO JÚNIOR, UNESP; DANIEL MOURA DE AGUIAR, UFMT; ERNA GEESSIEN KROON, UFMG. |
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Título: |
Equine infectious anemia virus in naturally infected horses from the Brazilian Pantanal. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Archives of Virology, v. 163, p. 2385-2394, 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
Equine infectious anemia (EIA) has a worldwide distribution, and is widespread in Brazil. The Brazilian Pantanal presents with high prevalence comprising equine performance and indirectly the livestock industry, since the horses are used for cattle management. Although EIA is routinely diagnosed by the agar gel immunodiffusion test (AGID), this serological assay has some limitations, so PCR-based detection methods have the potential to overcome these limitations and act as complementary tests to those currently used. Considering the limited number of equine infectious anemia virus (EIAV) sequences which are available in public databases and the great genome variability, studies of EIAV detection and characterization molecular remain important. In this study we detected EIAV proviral DNA from 23 peripheral blood mononuclear cell (PBMCs) samples of naturally infected horses from Brazilian Pantanal using a semi-nested-PCR (sn-PCR). The serological profile of the animals was also evaluated by AGID and ELISA for gp90 and p26. Furthermore, the EIAV PCR amplified DNA was sequenced and phylogenetically analyzed. Here we describe the first EIAV sequences of the 5? LTR of the tat gene in naturally infected horses from Brazil, which presented with 91% similarity to EIAV reference sequences. The Brazilian EIAV sequences also presented variable nucleotide similarities among themselves, ranging from 93,5% to 100%. Phylogenetic analysis showed that Brazilian EIAV sequences grouped in a separate clade relative to other reference sequences. Thus this molecular detection and characterization may provide information about EIAV circulation in Brazilian territories and improve phylogenetic inferences. MenosEquine infectious anemia (EIA) has a worldwide distribution, and is widespread in Brazil. The Brazilian Pantanal presents with high prevalence comprising equine performance and indirectly the livestock industry, since the horses are used for cattle management. Although EIA is routinely diagnosed by the agar gel immunodiffusion test (AGID), this serological assay has some limitations, so PCR-based detection methods have the potential to overcome these limitations and act as complementary tests to those currently used. Considering the limited number of equine infectious anemia virus (EIAV) sequences which are available in public databases and the great genome variability, studies of EIAV detection and characterization molecular remain important. In this study we detected EIAV proviral DNA from 23 peripheral blood mononuclear cell (PBMCs) samples of naturally infected horses from Brazilian Pantanal using a semi-nested-PCR (sn-PCR). The serological profile of the animals was also evaluated by AGID and ELISA for gp90 and p26. Furthermore, the EIAV PCR amplified DNA was sequenced and phylogenetically analyzed. Here we describe the first EIAV sequences of the 5? LTR of the tat gene in naturally infected horses from Brazil, which presented with 91% similarity to EIAV reference sequences. The Brazilian EIAV sequences also presented variable nucleotide similarities among themselves, ranging from 93,5% to 100%. Phylogenetic analysis showed that Brazilian EIAV sequences grouped in a sep... Mostrar Tudo |
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Palavras-Chave: |
PCR-based. |
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Thesagro: |
Anemia Infecciosa; Eqüino; Vírus. |
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Thesaurus Nal: |
Equine infectious anemia virus; Horses. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02510naa a2200277 a 4500
001 2099900
005 2018-11-30
008 2018 bl uuuu u00u1 u #d
100 1 $aCURSINO, A. E.
245 $aEquine infectious anemia virus in naturally infected horses from the Brazilian Pantanal.$h[electronic resource]
260 $c2018
520 $aEquine infectious anemia (EIA) has a worldwide distribution, and is widespread in Brazil. The Brazilian Pantanal presents with high prevalence comprising equine performance and indirectly the livestock industry, since the horses are used for cattle management. Although EIA is routinely diagnosed by the agar gel immunodiffusion test (AGID), this serological assay has some limitations, so PCR-based detection methods have the potential to overcome these limitations and act as complementary tests to those currently used. Considering the limited number of equine infectious anemia virus (EIAV) sequences which are available in public databases and the great genome variability, studies of EIAV detection and characterization molecular remain important. In this study we detected EIAV proviral DNA from 23 peripheral blood mononuclear cell (PBMCs) samples of naturally infected horses from Brazilian Pantanal using a semi-nested-PCR (sn-PCR). The serological profile of the animals was also evaluated by AGID and ELISA for gp90 and p26. Furthermore, the EIAV PCR amplified DNA was sequenced and phylogenetically analyzed. Here we describe the first EIAV sequences of the 5? LTR of the tat gene in naturally infected horses from Brazil, which presented with 91% similarity to EIAV reference sequences. The Brazilian EIAV sequences also presented variable nucleotide similarities among themselves, ranging from 93,5% to 100%. Phylogenetic analysis showed that Brazilian EIAV sequences grouped in a separate clade relative to other reference sequences. Thus this molecular detection and characterization may provide information about EIAV circulation in Brazilian territories and improve phylogenetic inferences.
650 $aEquine infectious anemia virus
650 $aHorses
650 $aAnemia Infecciosa
650 $aEqüino
650 $aVírus
653 $aPCR-based
700 1 $aVILELA, A. P. P.
700 1 $aFRANCO-LUIZ, A. P. M.
700 1 $aOLIVEIRA, J. G. de
700 1 $aNOGUEIRA, M. F.
700 1 $aARAÚJO JÚNIOR, J. P.
700 1 $aAGUIAR, D. M. de
700 1 $aKROON, E. G.
773 $tArchives of Virology$gv. 163, p. 2385-2394, 2018.
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Registro original: |
Embrapa Pantanal (CPAP) |
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| Registros recuperados : 16 | |
| 3. |  | DRUMOND, B. P.; LEITE, J. A.; FONSECA, F. G.; BONJARDIM, C. A.; FERREIRA, P. C. P.; KROON, E. G. Brazilian vaccinia virus strains are genetically divergent and differ from the lister vaccine strain. Microbes and Infection, Dorchester, v. 10, n. 2, p. 185-197, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Gado de Leite. |
|    |
| 4. | | LEITE, J. A.; DRUMOND, B. P.; TRINDADE, G. de S.; BONJARDIM, C. A.; FERREIRA, P. C. P.; KROON, E. G. Brazilian vaccinia virus strains show genetic polymorphism at the ati gene. Virus Genes, v. 35, n. 3, p. 531-539, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | CURSINO, A. E.; VILELA, A. P. P.; OLIVEIRA, D. B.; FERREIRA, P. C. P.; LIMA, M. F. N. T. de; LIMA, J. P. A.; KROON, E. G. Detecção e caracterização molecular do Equine Infectious Anemia virus em amostras de cavalos provenientes do pantanal brasileiro. In: SIMPÓSIO DE MICROBIOLOGIA DA UFMS, 2., 2015, Belo horizonte. Caderno de resumos... Belo Horizonte: [s.n.], 2015. p. 36.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 6. | | LEITE, J. A.; LEAL, A. do A.; RAMOS, J. G.; TRINDADE, G. de S.; ABRAHÃO, J. S.; SILVA, M. R.; KROON, E. G. Identificação sorológica e molecular do agente viral envolvido em surtos de vaccínia bovina no estado de Goiás. In: CONGRESSO INTERNACIONAL DO LEITE, 10.; WORKSHOP SOBRE POLÍTICAS PÚBLICAS, 10.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 11., 2011, Maceió. Anais... Juiz de Fora: Embrapa Gado de Leite, 2011. 3 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | MALOSSI, C. D.; CAVALCANTE, R. V.; ULLMANN, L. S.; NOGUEIRA, M. F.; AGUIAR, D. M.; KROON, E. G.; ARAUJO JR. J. P. Identification of a novel equine infectious anemia virus strain by massive sequencing in an endemic zone in Pantanal, Brazil. Journal of the Brazilian Society for Virology, v. 19, p.259-260, 2014. Supplement 2. BRAZILIAN CONGRESS OF VIROLOGY, 25.; MERCOSUR MEETING OF VIROLOGY, 9., 2014, Ribeirão Preto, SP.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 8. | | CURSINO, A. E.; LIMA, M. T.; NOGUEIRA, M. F.; AGUIAR, D. M. de; LUIZ, A. P. M. F.; ALVES, P. A.; ARAUJO JUNIOR, J. P.; KROON, E. G. Identification of large genetic variations in the equine infectious anemia virus tat-gag genomic region. Transboundary and Emerging Diseases, v. 68, n. 6, p. 3424-3432, 2021.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Pantanal. |
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| 9. | | MALOSSI, C. D.; FIORATTI, E. G.; CARDOSO, J. F.; MAGRO, A. J.; KROON, E. G.; AGUIAR, D. M. de; BORGES, A. M. C. M; NOGUEIRA, M. F.; ULLMANN, L. S.; ARAUJO JUNIOR, J. P. High genomic variability in Equine Infectious Anemia Virus obtained from naturally infected horses in Pantanal, Brazil: an endemic region case. Viruses, v. 12, n. 2, 207, p. 1-15, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pantanal. |
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| 10. | | CURSINO, A. E.; VILELA, A. P. P.; FRANCO-LUIZ, A. P. M.; OLIVEIRA, J. G. de; NOGUEIRA, M. F.; ARAÚJO JÚNIOR, J. P.; AGUIAR, D. M. de; KROON, E. G. Equine infectious anemia virus in naturally infected horses from the Brazilian Pantanal. Archives of Virology, v. 163, p. 2385-2394, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pantanal. |
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| 11. | | ABRAHÃO, J. S.; FERNANDES, A. T. S.; ASSIS, F. L.; GUEDES, M. I.; DRUMOND, B. P.; LEITE, J. de A.; COELHO, L. F. L.; TURRINI, F.; FONSECA, F. G.; LOBATO, Z. I. P.; MADUREIRA, M.; FERREIRA, P. C.; BONJARDIM, C. A.; TRINDADE, G. S.; KROON, E. G. Human vaccinia virus and pseudocowpox virus co-infection: clinical description and phylogenetic characterization. Journal of Clinical Virology, v. 48, p. 69-72, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 12. | | ABRAHÃO, J.; SILVA, L.; SILVA, L. S.; KHALIL, J. Y. B; RODRIGUES, R.; ARANTES, T.; ASSIS, F.; BORATTO, P.; ANDRADE, M.; KROON, E. G.; RIBEIRO, B.; BERGIER, I.; SELIGMANN, H.; GHIGO, E.; COLSON, P.; LEVASSEUR, A.; KROEMER, G.; RAOULT, D.; LA SCOLA, B. Tailed giant Tupanvirus possesses the most complete translational apparatus of the known virosphere. Nature Communications, v. 9, n. 749, p. 1-12, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pantanal. |
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| 13. | | ANDRADE, A. C. dos S. P.; ARANTES, T. S.; RODRIGUES, R. A. L.; MACHADO, T. B.; DORNAS, F. P.; LANDELL, M. F.; FURST, C.; BORGES, L. G. A.; DUTRA, L. A. L.; ALMEIDA, G.; TRINDADE, G. de S.; BERGIER, I.; ABRAHÃO, W.; BORGES, I. A.; CORTINES, J. R.; OLIVEIRA, D. B. de; KROON, E. G.; ABRAHÃO, J. S. Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica. Virology Journal, v. 15, n. 22, p. 1-10, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pantanal. |
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| 14. | | LEITE, J. de A.; FONSECA, F. G. da; TRINDADE, G. de S.; ABRAHÃO, J. S.; ARANTES, R. M. E.; ALMEIDA-LEITE, C. M. de; SANTOS, J. R. dos; GUEDES, M. I. M. C.; RIBEIRO, B. M.; BONJARDIM, C. A.; FERREIRA, P. C. P.; KROON, E. G. A-type inclusion bodies: a factor influencing cowpox virus lesion pathogenesis. Archives of Virology, n. 156, n. 4, p. 617-628, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 15. | | LEITE, J. de A.; FONSECA, F. G.; TRINDADE, G. S.; ABRAHÃO, J. S.; ARANTES, R. M. E.; LEITE, C. M. A.; SANTOS, J. R. DOS; GUEDES, M. I. M. C.; RIBEIRO, B. M.; BONJARDIM, C. A.; FERREIRA, P. C. P.; KROON, E. G. A-type inclusion body biological role: a factor influencing on cowpoxvirus lesion pathogenesis? In: NATIONAL MEETING OF VIROLOGY, 20., 2009, Brasilia. Anais... Brasília: SBV, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | MACHADO, T. B.; ANDRADE, A. C. dos S. P.; DORNAS, F. P.; MARINHO, P. E. S.; REIS, E. V. de S.; SILVA, N. I. O. da; AQUINO, I. L. M. de; MENDONÇA, D. C.; DRUMOND, B. P.; BORATTO, P. V. de M.; PICORELLI, A. C. R.; FIGUEIREDO, P. de O.; ANDRADE, A. de S.; ARANTES, T. S.; BERGIER, I.; COMERLADO, J.; KROON, E. G.; ABRAHÃO, J. S. Prospecção de vírus gigantes em biomas brasileiros. In: SIMPÓSIO DE MICROBIOLOGIA DA UFMG, 5., 2018, Belo Horizonte. Resumos... Belo Horizonte: Universidade Federal de Minas Gerais, 2018. p. 151.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 16 | |
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